Enhancement of Staining Intensity of Mosquito Larva Zymograms after Electrophoresis
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Silver staining of 2D electrophoresis gels.
Silver staining is used to detect proteins after electrophoretic separation on polyacrylamide gels. It -combines excellent sensitivity (in the low nanogram range) with the use of very simple and cheap equipment and chemicals. For its use in proteomics, two important additional features must be considered, compatibility with mass spectrometry and quantitative response. Both features are discusse...
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The formed elements of human blood each contain multiple isoenzymes of nonspecific esterase that hydrolyze short chain alpha naphthyl esters. Zymograms that are characteristic of each type of formed element are obtained by subjecting purified preparations of each to polyacrylamide slab gel electrophoresis at pH 9.5 and subsequent staining of the gels for esterase activity. The most prominent is...
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ژورنال
عنوان ژورنال: Journal of UOEH
سال: 1989
ISSN: 0387-821X,2187-2864
DOI: 10.7888/juoeh.11.461